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phycoerythrin pe conjugated mouse anti human p selectin antibody  (R&D Systems)


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    R&D Systems phycoerythrin pe conjugated mouse anti human p selectin antibody
    Phycoerythrin Pe Conjugated Mouse Anti Human P Selectin Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+conjugated+mouse+anti+human+e+selectin/Human+E-Selectin%2FP-Selectin+(CD62E%2FP)+PE-conjugated+Antibody/pm29322294-37-20-26
    Average 90 stars, based on 4 article reviews
    phycoerythrin pe conjugated mouse anti human p selectin antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: Bacterial lipoprotein Toll-like receptor 2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivo
    Article Snippet: The cells were then washed using Flow Cytometry Staining Buffer (FCSB, R&D Systems) and then were incubated with 10 μg of human IgG (R&D Systems) in 0.2 mL of FCSB for 15 minutes at 4°C. .. After washing twice with FCSB, the cells were incubated for 45 minutes at 4°C with primary antibodies, which included unconjugated goat anti-human TLR2 and normal goat IgG control (CD282, R&D Systems and EMD Gibbstown, NJ respectively; 2 μg/sample) and PE-conjugated mouse anti-human E-selectin and normal mouse IgG1 control (CD62-PE and mouse IgG1–PE, R&D Systems; 1:10 dilution). .. The TLR2 samples and controls were then washed with FCSB and incubated with FITC-conjugated secondary antibody (donkey-α-goat-FITC, Millipore, Billerica, MA; 2 μg/sample) for 45 minutes at 4°C.

    Control:

    Article Title: Bacterial lipoprotein Toll-like receptor 2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivo
    Article Snippet: The cells were then washed using Flow Cytometry Staining Buffer (FCSB, R&D Systems) and then were incubated with 10 μg of human IgG (R&D Systems) in 0.2 mL of FCSB for 15 minutes at 4°C. .. After washing twice with FCSB, the cells were incubated for 45 minutes at 4°C with primary antibodies, which included unconjugated goat anti-human TLR2 and normal goat IgG control (CD282, R&D Systems and EMD Gibbstown, NJ respectively; 2 μg/sample) and PE-conjugated mouse anti-human E-selectin and normal mouse IgG1 control (CD62-PE and mouse IgG1–PE, R&D Systems; 1:10 dilution). .. The TLR2 samples and controls were then washed with FCSB and incubated with FITC-conjugated secondary antibody (donkey-α-goat-FITC, Millipore, Billerica, MA; 2 μg/sample) for 45 minutes at 4°C.



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    Analysis of the adhesion strength of EPCs to activated endothelium using single-cell atomic force microscopy (AFM). Representative histograms of the adhesion signature of CXCR4 and PSGL-1 mRNA (mRNA cocktail) modified and native EPCs to TNF-α-activated and nonactivated endothelium and the quantification of adhesion forces are shown. Bonds formed between the cell surface ligands (PSGL-1) and molecules on HUVECs (E-selectin) broke slightly with increasing force until the cell completely detached from the HUVEC. The maximum downward force of the cantilever’s tip is referred to as detachment force (F detach ). ( n = 4). Statistical analysis was performed using two-way ANOVA, followed by Bonferroni´s multiple comparisons test (** p < 0.01 and **** p < 0.0001).

    Journal: Pharmaceutics

    Article Title: Homing of mRNA-Modified Endothelial Progenitor Cells to Inflamed Endothelium

    doi: 10.3390/pharmaceutics14061194

    Figure Lengend Snippet: Analysis of the adhesion strength of EPCs to activated endothelium using single-cell atomic force microscopy (AFM). Representative histograms of the adhesion signature of CXCR4 and PSGL-1 mRNA (mRNA cocktail) modified and native EPCs to TNF-α-activated and nonactivated endothelium and the quantification of adhesion forces are shown. Bonds formed between the cell surface ligands (PSGL-1) and molecules on HUVECs (E-selectin) broke slightly with increasing force until the cell completely detached from the HUVEC. The maximum downward force of the cantilever’s tip is referred to as detachment force (F detach ). ( n = 4). Statistical analysis was performed using two-way ANOVA, followed by Bonferroni´s multiple comparisons test (** p < 0.01 and **** p < 0.0001).

    Article Snippet: After 4 h, HUVECs with or without TNF-α treatment were stained with 5 µL mouse anti-human CD62E (E-selectin)-PE-conjugated antibody (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions, and the E-selectin expression was analyzed using flow cytometry.

    Techniques: Microscopy, Modification

    Dynamic adhesion of mRNA-modified EPCs to activated endothelium. ( A ) HUVECs were treated with 10 ng/mL TNF-α, and E-selectin expression was analyzed by flow cytometry to analyze the successful activation of the cells. ( n = 3). Statistical analysis was performed using a t -test (* p < 0.05 and *** p < 0.001). ( B ) EPCs were transfected with an mRNA cocktail of CXCR4 and PSGL-1 mRNA (1 µg each). After 24 h, the cells were stained with PKH26 and perfused over TNF-α-activated HUVECs in a flow chamber at 0.11 mL/min and a shear stress of 0.1 dyn/m 2 . ( B ) Representative images of rolling EPCs are shown every 2 s. Red arrows indicate a slowly rolling cell, and yellow arrows point to a fast-moving cell not interacting with the activated HUVECs. ( C ) Representative images of recordings at 1, 3, 5, and 7 min after starting the flow. ( D ) The number of EPCs adhered to HUVECs after 7 min was quantified ( n = 3). Statistical analysis was performed using two-way ANOVA, followed by Bonferroni´s multiple comparisons test (** p < 0.01).

    Journal: Pharmaceutics

    Article Title: Homing of mRNA-Modified Endothelial Progenitor Cells to Inflamed Endothelium

    doi: 10.3390/pharmaceutics14061194

    Figure Lengend Snippet: Dynamic adhesion of mRNA-modified EPCs to activated endothelium. ( A ) HUVECs were treated with 10 ng/mL TNF-α, and E-selectin expression was analyzed by flow cytometry to analyze the successful activation of the cells. ( n = 3). Statistical analysis was performed using a t -test (* p < 0.05 and *** p < 0.001). ( B ) EPCs were transfected with an mRNA cocktail of CXCR4 and PSGL-1 mRNA (1 µg each). After 24 h, the cells were stained with PKH26 and perfused over TNF-α-activated HUVECs in a flow chamber at 0.11 mL/min and a shear stress of 0.1 dyn/m 2 . ( B ) Representative images of rolling EPCs are shown every 2 s. Red arrows indicate a slowly rolling cell, and yellow arrows point to a fast-moving cell not interacting with the activated HUVECs. ( C ) Representative images of recordings at 1, 3, 5, and 7 min after starting the flow. ( D ) The number of EPCs adhered to HUVECs after 7 min was quantified ( n = 3). Statistical analysis was performed using two-way ANOVA, followed by Bonferroni´s multiple comparisons test (** p < 0.01).

    Article Snippet: After 4 h, HUVECs with or without TNF-α treatment were stained with 5 µL mouse anti-human CD62E (E-selectin)-PE-conjugated antibody (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions, and the E-selectin expression was analyzed using flow cytometry.

    Techniques: Modification, Expressing, Flow Cytometry, Activation Assay, Transfection, Staining